The Immunomodulatory Effects of Umbilical Cord Mesenchymal Stem Cell in Critical Limb Ischemia Patients

Transplantation of human umbilical cord mesenchymal stem cells (UC-MSCs) has been shown to be effective in treating critical limb ischemia (CLI). However, the mechanism of MSCsmediated improvements in CLI patients, especially with respect to the immuneinflammatory aspects of this disease, is still unknown. In this study, we investigated the changes in T-lymphocyte subpopulations and inflammatory mediators (such as IL-6, IL-10 and TNF-α) in PBMCs from CLI patients after UC-MSCs treatment, as well as the correlation between inflammatory mediators and EPCs. TNF-α serum levels increased at 24 h (p=0.017) after treatment and then decreased at 1 month (p=0.031) compared with that before treatment. IL6 serum levels increased at 24 h (p=0.099) after treatment and then decreased at 1 month (p=0.072) compared with that before treatment. The percentages of CD3+ T, CD3+ CD4+ lymphocytes and NK cells were significantly decreased after UC-MSCs treatment (p=0.002, p=0.012 and p=0.029, respectively). TNF-α (r=-0.42, p=0.0004) and IL-6(r=-0.33, P < 0.0001) were shown to be inversely correlated with the number of circulating EPCs. Our studies have shown that UC-MSCs have anti-inflammatory and immunomodulation properties in CLI and promotes healing of non-healing wounds. The Immunomodulatory Effects of Umbilical Cord Mesenchymal Stem Cell in Critical Limb Ischemia Patients Wen-Hui Gao, Jing-Yi Yu, Hong-Min Li, Yue Guan, Shang-Zhu Li and Ping-Ping Huang* Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China *Corresponding author: Ping-Ping Huang, Doctor of Medicine, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China, Tel: 13612018426; Fax: 02223909058; E-mail: [email protected] Received June 28, 2016; Accepted July 02, 2016; Published July 08, 2016 Citation: Gao WH, Yu JY, Li HM, Guan Y, Li SZ, et al. (2016) The Immunomodulatory Effects of Umbilical Cord Mesenchymal Stem Cell in Critical Limb Ischemia Patients. J Stem Cell Res Ther 6: 349. doi:10.4172/2157-7633.1000349 Copyright: © 2016 Gao WH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.